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1.
Transglutaminase (TGase) was separated from the culture broth of an isolated strain of Streptoverticillium mobaraense. The crude enzyme was prepared by centrifugation, ultrafiltration, precipitation by alcohol, centrifugation and freeze‐drying. The yield after these processes was 65–70%. Then the enzyme was purified to homogeneity by chromatography on CM‐cellulose and Sephadex G‐75 on which the yields were about 70% and 80%, respectively; the purified folds reached 2.5–4.7 and 1.08–2.06, respectively. The molecular weight of this TGase was 39,500–40,100 Da by gel filtration chromatography. Optimum enzyme activity was observed in the pH range of 5.0–7.0, and it was maintained stable at 20–40C. The optimal temperature and pH was 52C and 6.0, respectively. At 1 mM and 5 mM metal ion or inhibitors concentration, TGase activity was strongly inhibited by Zn2+ and NEM, and not affected obviously by Ba2+, Ca2+, Co2+, Cu2+, Fe2+, Fe3+, Mg2+, Mn2+, Na+ as well as PMSF and EDTA. The effects of these additions on this TGase were compared with those of other microbial TGases. 相似文献
2.
基于FPGA乘法器架构的RNS与有符号二进制量转换 总被引:1,自引:1,他引:0
RNS(余数数制系统)是一种整数运算系统,在粒度精确性,能源损耗和响应速度上有很大的优势.从RNS到二进制数的输入输出转换是基于余数算法的专用架构实现的关键.本文提出了一个基于N类模的RNS与有符号二进制量的通用转换算法在FPGAs的乘法器上的实现过程.该算法能更有效地进行有符号数与RNS的转换.基于该算法类型乘法器在同类型乘法器中显示出了速度优势.文章中该架构被映射到Altera的10K系列的FPGA上. 相似文献
3.
《Thin solid films》2006,515(2):782-785
We present theoretical photoluminescence (PL) and absorption spectra of p-doped InGaN/AlInGaN and AlInGaN/InGaN multiple quantum wells (MQWs). The calculations were performed within the k.p method by means of solving a full eight-band Kane Hamiltonian together with the Poisson equation in a plane wave representation, including exchange–correlation effects within the local density approximation. Strain effects due to the lattice mismatch and an internal electric field are also taken into account. We show that by changing the In and Al composition we can reach short and long emission wavelengths. The trends in the calculated Stokes shift, due to many-body effects within the quasi-two-dimensional hole gas (2DHG), are analyzed as a function of the acceptor doping concentration. Since the studies of optical properties of quantum wells based on nitrides quaternary alloys are at an early stage, the results reported here will provide guidelines for the interpretation of forthcoming experiments. 相似文献
4.
Alleviation Effect of Lanthanum on Cadmium Stress in Seedling Hydroponic Culture of Kidney Bean and Corn 总被引:4,自引:0,他引:4
Since1970s,rare earths(RE)have been exten-sively used as micro-fertilizers for crops in China.Ithas already been proved that REcani mprove the pro-duction and quality of crops.However,there havebeen a fewinvestigations about using RE as regulatorin pollution ecology.For example,An et al[1]studiedthe effects of O3on wheat growth and the protectiveeffect of RE.Yan and Zhou et al[2,3]discussed theprotective effect of RE on plants under acid rainstress.Jia et al[4]and Hu et al[5]reported th… 相似文献
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6.
COMPARATIVE STUDY OF CHEMICAL COMPOSITION AND PHYSICOCHEMICAL PROPERTIES OF DEFATTED WHEAT GERM FLOUR AND ITS PROTEIN ISOLATE 总被引:2,自引:0,他引:2
Chemical composition and physicochemical properties of defatted wheat germ (WG) flour (DWGF) and protein isolate (DWGPI) were evaluated to determine their potential as food ingredients. Protein content of DWGF was 31.42% while that of DWGPI was 84.18%. Comparison of the amino acid composition to the Food and Agriculture Organization/World Health Organization reference pattern indicated that DWGF and DWGPI had well‐balanced amino acid profiles. Both of them were relatively rich in lysine, whereas cystine was lacking. The main mineral constituents of DWGF were potassium, magnesium and calcium, while sodium was the dominating element in DWGPI as a result of salt extraction. Concentrations of potassium, magnesium, calcium, manganese and zinc in DWGPI were significantly decreased because of alkaline extraction compared to those in DWGF. The elements copper and iron were higher in DWGPI than in DWGF. Total flavonoid content of DWGF was about 0.35 g rutin equiv/100 g, whereas that of DWGPI was lower (about 0.24 g rutin equiv/100 g). Compared to soybean protein isolate, DWGPI was easily digested by pepsin in vitro, while DWGF was comparatively indigestible. Nonreduced and reduced sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analyses seem to show that interpolypeptide S‐S bonds were absent in the structure of WG proteins. DWGF and DWGPI shared four groups of bands: (1) 85–70 kDa; (2) 55–50 kDa; (3) 47–40 kDa; and (4) below 20 kDa. 相似文献
7.
依据概率密度逼近提出了一种新的无监督特征排序,应用于特征选择降维.实验证明,这种方法与一些现有的方法相比,更为有效. 相似文献
8.
QPSO算法在非线性观测器设计中的应用* 总被引:1,自引:0,他引:1
具有量子行为的粒子群优化(Quantum-behaved Particle Swarm Optimization,QPSO)算法是继粒子群优化算法(Particle Swarm Optimization,PSO)后,最新提出的一种新型、高效的进化算法.提出了运用QPSO算法设计的非线性观测器方法.该方法属于滚动时域估计方法,利用具有量子行为的粒子群算法优化获得系统状态的最优估计.仿真结果显示该方法对初始条件不敏感,具有很强的跟踪能力. 相似文献
9.
本文介绍了TFC?-S型纳滤膜分离电镀镍漂洗水的试验研究,讨论了温度、操作压力、浓差极化、共存离子对膜分离性能的影响。结果表明,这种纳滤膜对电镀镍漂洗水中Ni2 的去除率高于99.5%,透过液中Ni2 质量浓度小于1mg/L;对CODCr的去除率大于96%,透过液中CODCr低于14mg/L,达到国家工业废水排放标准(GB8978-1996),可以直接排放或回用于镀件漂洗。 相似文献
10.